Human DPP8 (Dipeptidylpeptidase 8), GenBank Accession No. NM_197960, full length with N-terminal GST-tag, MW=129.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Specific activity: >= 560 pmol/min/ug
Background
Dipeptidyl peptidase-8 (DPP8) is a ubiquitously expressed serineexopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.DPP8 shares homology and substrate recognition pattes with DPP4 and like DPP4, isthought to play a role in T-cell activation and immune function.
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 18 mM Glutathione,10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM Tris (pH 7.4),0.1% BSA, 10 uM Ala-Pro-AMC, 10 min.at room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_197960
Mw(kda)
129.3 kDa
Synonyms
Dipeptidylpeptidase 8, MSTP141
Uniprot
Q6V1X1
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Lee, H.J. et al., J. Biol. Chem. 281 (50),38653-38662 (2006). 2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460(2006).