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Home / Proteins & Peptide

DNMT3B/DNMT3L Complex, GST-tag, His-tag

SKU : BHP18201816

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BPS Bioscience

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Mfr.No.	51109
Description	Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal GST-tag, MW=59 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal His-tag, MW = 27 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system. host species: insect Host species: insect Host cell line: Sf9 Specific activity: >=0.01 pmol/min/ug.
Immunogen Region	564-853(end), DNMT3b; 160-387(end), DNMT3L
Tag	N-terminal GST (DNMT3B) N-terminal His-tag (DNMT3L)
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerol
Species	Human
Application	Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Notes	Assayed using Biohippo DNMT3B Assay Kit, Cat. #52034. Add 50 ul reaction mixcontaining DNMT3B/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection. Avoid freeze/thaw cycles.
GenBank	NM_006892(DNMT3B), NM_013369 (DNMT3L)
Mw(kda)	86 kDa (complex)

Uniprot	Q9UBC3 (DNMT3B), Q9UJW3 (DNMT3L)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201816	51109	10 ug	$522.50		Add to Cart

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