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Home / Proteins & Peptide

DNMT3A/DNMT3L Complex, His-tag, GST-tag Recombinant

SKU : BHP18201815

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BPS Bioscience

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Mfr.No.	51106
Description	Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system. host species: insect Host species: insect Host cell line: Sf9 Specific activity: >=0.58 pmol/min/ug.
Immunogen Region	2-1632
Tag	N-terminal His, N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
Species	Human
Application	Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Notes	Assayed using Biohippo DNMT3A Assay Kit, Cat. #52033. Add 50 ul reaction mixcontaining DNMT3A/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection. Avoid freeze/thaw cycles.
GenBank	NM_175629(DNMT3A), NM_013369 (DNMT3L)
Mw(kda)	86 kDa (complex)

Uniprot	Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201815	51106	10 ug	$522.50		Add to Cart

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