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Home / Proteins & Peptide

DNMT1, His-FLAG-tags

SKU : BHP18200437

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BPS Bioscience

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Mfr.No.	51100
Description	Recombinant human DNMT1 (DNA methyltransferase 1, isofrom b), encompassing amino acids 501-1632 (end). This construct contains an N-terminal His-tag (6xHis) followed by a FLAG tag. The protein was affinity purified. host species: insect Host species: insect Host cell line: Sf9 Specific activity: >=0.16 pmol/min/ug.
Immunogen Region	501-1632 (end)
Tag	N-terminal His-FLAG-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/ul FLAG peptide, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed using the DNMT1 Assay kit, BPS Cat. #52050L, using 40 uM S-adenosylmethioninefor 1 hour at 37°C. Avoid freeze/thaw cycles.
GenBank	NM_001130823
Mw(kda)	130 kDa

Synonyms	TRDMT1
Uniprot	P26358-2

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Tan, H.H. and Porter, A.G. Biochem. Biophys. Res. Commun. 382 (2), 462-467 (2009). 2. Fan, H. et al., World J. Gastroenterol. 15 (16), 2020-2026 (2009).Application Reference(s): 1. Antroquinonol D, isolated from Antrodia camphorata, with DNA demethylation and anticancer potential (2014) 2. DNA 5-methylcytosine demethylation activities of the mammalian DNA methyltransferases (2013) 3. DNMT1-interacting RNAs block gene-specific DNA methylation (2013) 4.Electrochemical assay for the signal-on detection of human DNA methyltransferase activity (2013) 5.Methylation of RASSF1A gene promoter is regulated by p53 and DAXX (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200437	51100	20 ug	$478.50		Add to Cart

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