Recombinant human DNMT1 (DNA methyltransferase 1, isoform b ), full-length, encompassing amino acids 2-1632(end). This construct contains an N-terminal GST-tag and includes a thrombin cleavage site to facilitate removal of the GST portion. The recombinant protein was affinity purified.
host species: insect
Host species: insect
Host cell line: Sf9
Immunogen Region
2-1632
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performedusing the DNMT1 Assay kit, BPS Cat. #52050L, using 20 uM S-adenosyl-methioninefor 1 hour at 37°C.