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Home / Proteins & Peptide

DEP1(PTPRJ), GST-tag

SKU : BHP18200043

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Mfr.No.	30050
Description	Protein tyrosine phosphatase DEP1 (density-enhanced phosphatase-1), also known as PTPRJ, PTP-eta and CD148 (GenBank accession no. NM_U10886), a.a. 1024-1306 (catalytic domain) with N-terminal GST-tag, MW=58.9 kDa, expressed in an E. coli expression system. Specific activity: 268 nmole/min/ug
Background	Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Positive regulator of platelet activation and thrombosis. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation through KDR dephosphorylation. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration through dephosphorylation of PDGFR. Negative regulator of EGFR signaling pathway via EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
Immunogen Region	1024-1306
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Species	Human
Application	Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Notes	20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30°C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on epsilon_pNPP= 18,000 M^-1cm^-1. Avoid freeze/thaw cycles.
GenBank	NM_002843
Mw(kda)	58.9 kDa

Synonyms	DEP1, density-enhanced phosphatase-1, PTP-eta, CD148, PTPRJ
Uniprot	Q12913

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	>12 months at -80°C

Reference	1. A. Massa, et al., J Biol Chem. 2004, 279: 29004-12. 2. S. Kellie, et al., J Cell Sci. 2004, 117 :609-18.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200043	30050	50 ug was 20 ug	$357.50		Add to Cart

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