Human secreted CTLA4, Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36- 162, fused at the C-terminus to the Fc portion of human IgG1 and with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein was biotinylated using AviTag technology. MW = 42 kDa (monomer). Note: This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
36-162
Tag
C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Formulation
40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for studying protein binding and screening small molecular inhibitors
Notes
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_005214
Mw(kda)
42 kDa + glycans
Synonyms
Cytotoxic T-lymphocyte-associated protein 4, CD152
Uniprot
P16410
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Dilek, N., et al., PLOS ONE. 2013 Dec. 23; 8(12):e83139. 2. Leach, D.R., et al., Science. 1996; 271(5256):1734-1736.