Human CSK (GenBank Accession No. NM_004383), full length with N-terminal GST-tag, MW= 80 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 100 U/mg.
Unit definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, 10 mM reduced glutathione, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na orthovanadate, 0.1 mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 1 ug/ml recombinant CSK.
Avoid freeze/thaw cycles.
GenBank
NM_004383
Mw(kda)
80 kDa
Synonyms
CSK, C-terminal Src kinase
Uniprot
P41240
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Manjarrez-OrduA±o N, et al. Nat Genet. 2012 Nov,44(11):1227-30. 2. de la Puerta ML, et al. PLoS One. 2013,8(1):e54569.