Human FMS, also known as Colony stimulating factor 1 receptor (CSF1R) and CD115 (GenBank Accession No. BC047521), a.a. 538-end with N-terminal GST-tag, MW=75 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 24 pmol/min/ug
Immunogen Region
538-end
Tag
N-terminal GST-tag
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25%glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay buffer: 5 mM MOPS, 2.5 mM -glycerophosphate, 5 mM MgCl2, 1 mMEGTA, 0.4 mM EDTA, 0.05 mM DTT, 2mM ATP. Incubate BMX with 0.25mg/ml Poly (Glu:Tyr, 4:1) peptidesubstrate and (33P)-ATP at 30°C for 15minutes, then spot reaction onphosphocellulose paper, fix in 1%phosphoric acid, and assay with ascintillation counter.