Human RAF1, also known as c-RAF (GenBank Accession No. NM_002880), a.a. 306-648, containing the mutations Y340D and Y341D, with N-terminal GST-tag, MW=65 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 158 pmol/min/ug
Immunogen Region
306-648
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 uM ATP, 1 uM MEK1(K97R), 1 hour. ATP reduction was detected using ADP-Glo Kinase Assay Platform (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_002880
Mw(kda)
65 kDa
Synonyms
cRAF, RAF1, NS5, RAF-1
Uniprot
P04049
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Lamberti,A. et al. Cell Death Differ. 14 (5), 952-962 (2007). 2. Yu,F. et al. PLoS Pathog. 3 (3), E44 (2007).