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Home / Proteins & Peptide

COX2, FLAG-His-tags

SKU : BHP18200585

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BPS Bioscience

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Mfr.No.	71111
Description	Human COX2, GenBank Accession No. NM_000963, a.a. 1-604 (end), with C-terminal FLAG-His-tag, MW=71 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Immunogen Region	1-604 (end)
Tag	C-terminal FLAG-His-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 ug/ml FLAG peptide, 300 uM diethyldithiocarbamate and 0.08% Triton X-100.
Species	human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 uM EDTA) with 5 uM Hematin for 10 min then added 100 M Ampliflu Red and 200 uM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 ul. Avoid freeze/thaw cycles.
GenBank	NM_000963
Mw(kda)	71 kDa

Synonyms	Cyclooxygenase-2 Prostaglandin-Endo-peroxide Synthase 2, Prostaglandin G/H Synthase and Cyclooxygenase, PTGS2, Prostaglandin H2 Synthase, PHS-2, HCox-2, Inducible Prostaglandin H Synthase 2
Uniprot	P35354

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kosaka, T., et al., Eur J Biochem. 1994 May 1;221(3):889-97. 2. Cox, D.G., et al., Br J Cancer. 2004 Jul 19;91(2):339-43.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200585	71111	100 ug	$627		Add to Cart

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