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Home / Proteins & Peptide

COX1, FLAG-His-tags

SKU : BHP18201545

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BPS Bioscience

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Mfr.No.	71110-1
Immunogen Region	1-599 (end)
Tag	C-terminal FLAG-His-tags
Description	Human COX1, GenBank Accession No. NM_000962, a.a. 1-599 (end), with C-terminal FLAG-His-tag, MW=70.5 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 ug/ml FLAG peptide, 300 uM diethyldithiocarbamate and 0.08% Triton X-100.
Species	human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 uM EDTA) with 5 uM Hematin for 10 min then added 100 M Ampliflu Red and 200 uM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 ul. Avoid freeze/thaw cycles.
GenBank	NM_000962
Mw(kda)	70.5 kDa

Synonyms	Cyclooxygenase-1, Cox-1, Prostaglandin-Endo-peroxide Synthase 1, Prostaglandin G/H Synthase and Cyclooxygenase, PTGS1, PES-1, PGHS-1, Prostaglandin H2 Synthase 1, PTGHS
Uniprot	P23219

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Scott, B.T., et al., Blood Coagul Fibrinolysis. 2002 Sep;13(6):519-31. 2. Funk, C.D., et al., FASEB J. 1991 Jun;5(9):2304-12.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201545	71110-1	100 ug	$627		Add to Cart

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