Human COX1 protein, GenBank Accession No. NM_000962, a.a. 1-599 (end), with C-terminal FLAG-His-tag, MW=70.5 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Immunogen Region
1-599 (end)
Tag
C-terminal FLAG-His-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 ug/ml FLAG peptide, 300 uM diethyldithiocarbamate and 0.08% Triton X-100.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Mixed various concentrations (0-200 nM) of enzyme in Assay buffer (100 mM Tris-HCl ph 8, 3 uM EDTA) with 5 uM Hematin for 5 min then added 100 uM Ampliflu Red and 50 uM arachidonic acid (Cayman Chemical # 90010.1 peroxide free) and measured fluorescence (ex 535 em 590) after 5 min. Reaction volume was 100 ul.