Human core histones (twocopies each of H2A, H2B, H3, and H4),purified from cultured HeLa cells. Histonesare separated from DNA under high saltconditions.
host species: human
Host species: human
Host cell line: HeLa cells
Formulation
50 mM sodium phosphatebuffer, pH 6.8 and 2.5 M NaCl
Species
Human
Application
Ideal as a substrate for methylation or acetylation assays or for screening small molecule inhibitors of epigenetic enzymes in drug discovery and HTS applications.
Notes
Avoidfreeze/thaw cycles.
Shipping Temperature
-80°C (dry ice)
Format
An aqueous solution; sufficient for 50 reactions in a 96- well format
Storage
At least 6 months at -80°C.
Reference
1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11. 2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9. 3. Zhang, Y., et al., Genes and Dev. 2001; 15: 2343-2360.