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Home / Proteins & Peptide

Claudin-18 Isoform 2, FLAG-Tag Recombinant

SKU : BHP18201484

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BPS Bioscience

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Mfr.No.	101570-1
Description	Recombinant human Claudin-18, Isoform 2 (CLDN 18), full length, encompassing amino acids 1-261(end). This construct contains a C-terminal Flag-tag. The recombinant protein was affinity purified. Constructs: Claudin-18 Isoform 2 (1-261(end)-FLAG) host species: HEK293 Host species: HEK293
Immunogen Region	1-261(end)
Tag	C-terminal FLAG-tag
Formulation	50 mM HEPES, pH 8, 500 mM NaCl, 10% glycerol, 0.1% DDM, 0.01% CHS, and 100 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	The protein was validated by measuring its binding to Anti-Claudin-18 Isoform 2 IgG Antibody (Biohippo #101564) in biochemical binding (ELISA) assay. The Claudin-18 Isoform 2 protein was coated onto a 96-well plate overnight at 4°C (50 ul/well at a concentration of 2 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (Biohippo #79311) and blocked using 100 ul of Blocking Buffer 2 (Biohippo #79728) with DDM/CHS detergent (dodecyl-beta-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified Anti-Claudin-18 Isoform 2 IgG Antibody (Biohippo #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with HRP Colorimetric Substrate (Biohippo #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values. Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
GenBank	NM_001002026
Mw(kda)	29 kDa

Synonyms	Claudin-18, CLDN18, UNQ778/PRO1572
Uniprot	P56856

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201484	101570-1	10 ug	$324.50		Add to Cart

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