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Home / Proteins & Peptide

cKit (V654A), His-tag

SKU : BHP18200204

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Mfr.No.	40251
Description	Human Kit (V654A) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of valine654 to alanine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 89 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.
Immunogen Region	544-976(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant cKit. Assay was done with 0.2 mg/ml Poly-(Glu-Tyr) peptide using ADPGlo (Promega) conditions at 30°C for 35 minutes. Avoid freeze/thaw cycles.
GenBank	NM_000222
Mw(kda)	53.4 kDa

Synonyms	KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor
Uniprot	P10721

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114. 2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200204	40251	10 ug	$390.50		Add to Cart

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