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Home / Proteins & Peptide

cKit (D816V), His-tag

SKU : BHP18200205

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Mfr.No.	40252
Description	HumancKit (D816V) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of aspartic acid 816 to valine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 397 pmol/min/ug.
Immunogen Region	544-976(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate (Tyr Peptide 6, Invitrogen) at 30°C for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_000222
Mw(kda)	53.4 kDa

Synonyms	KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor
Uniprot	P10721

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114. 2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200205	40252	10 ug	$390.50		Add to Cart

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