Human Checkpoint kinase 2 (GenBank Accession No. NM_007194), full length, with N-terminal His-tag. MW=91 kDa. Expressed in a Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 6.9 pmoles/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 40% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
CHEK2 was incubated with a substrate for 1h at RT in kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Avoid freeze/thaw cycles.
GenBank
NM_007194
Mw(kda)
91 kDa
Synonyms
CHK2, CHEK2, checkpoint kinase 2 , CDS1, RAD53
Uniprot
O96017
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
>12 months at -80°C
Reference
1. Reinhardt HC and Yaffe MB. Curr Opin Cell Biol. 2009 Apr;21(2):245-55. 2. Stracker TH and Petrini JH. Cell Cycle. 2008 Dec,7(23):3618-21.