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Home / Proteins & Peptide

CERK, FLAG-Tag (Rat)

SKU : BHP18200753

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BPS Bioscience

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Mfr.No.	79414
Description	Rat CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system. Specific activity: >=20 pmole/min/ug
Immunogen Region	1-531 (end)
Tag	C-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 100ug/ml flag peptide
Species	Rat
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	CERK activity was measured at 37°C for 25 min in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 uM ATP) using a u-Octylglucoside mixed micellar assay with 100 uM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo Luminescent Kinase Assay Platform (Promega, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_00113486
Mw(kda)	61 kDa

Synonyms	ceramide kinase

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at 80°C. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.

Reference	1. Sugiura, M. et al., JBC. 277, 23294-23300 (2002)2. Majjalieh, S.M., et al., JBC. 264, 14354-14360 (1989)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200753	79414	10 ug	$467.50		Add to Cart

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