Mouse CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system.
Specific activity: >=10 pmole/min/ug
Immunogen Region
1-531 (end)
Tag
C-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 100ug/ml flag peptide
Species
Mouse
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay conditions: CERK activity was measured at 37°C for 25 min in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 uM ATP) using a u-Octylglucoside mixed micellar assay with 100 uM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo Luminescent Kinase Assay Platform (Promega, Madison, WI).
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_00113486
Mw(kda)
61 kDa
Synonyms
ceramide kinase
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Sugiura, M. et al., JBC. 277, 23294-23300 (2002)2. Majjalieh, S.M., et al., JBC. 264, 14354-14360 (1989)