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Home / Proteins & Peptide

Ceramide Kinase, FLAG-tag

SKU : BHP18200277

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BPS Bioscience

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Mfr.No.	40617
Description	Human ceramide kinase (CERK), GenBank Accession No. NM_022766, a.a. 1-537(end) with C-terminal FLAG-tag, MW= 60 kDa, expressed in a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 142 pmole/min/ug Unit definition: A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.
Immunogen Region	1-537(end)
Tag	C-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 ug/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl₂, 10 mM MgCl₂, 2 mM DTT, 5 uM ATP) using a beta-Octylglucoside mixed micellar assay with 100 uM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo Luminescent Kinase Assay Platform (Promega, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_022766
Mw(kda)	60 kDa

Synonyms	CERK, Ceramide Kinase, Lipid kinase 4, LK4, Acylsphingosine Kinase
Uniprot	Q8TCT0

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Mitra,P. et al., FEBS Lett. 581 (4), 735-740 (2007) 2. Kim,T.J. et al., Biochem. Biophys. Res. Commun. 342 (2), 611-617 (2006)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200277	40617	10 ug	$467.50		Add to Cart

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