Human Bromodomain CECR2 (cat eye syndrome chromosome region, candidate 2), GenBank Accession No. NM_031413, a.a. 430-543 corresponding to single bromodomain with N-terminal His-tag, MW = 14.4 kDa, expressed in an E. coli expression system.
Immunogen Region
430-543
Tag
N-terminal His-tag
Formulation
40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Species
Human
Application
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes
CeCR2 was incubated with various amounts of BET ligand in assay buffer in a 10 ul reaction for 30 min at RT. Reactions with H4 1-21(B) were prepared in parallel as control. AlphaLISA Ni acceptor beads (1:250) were added and incubation continued for 30 min more. Finally, Streptavidin Donor beads (1:125) were added and mixture was incubated for 10 min at RT before A-counts were measured.