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Home / Proteins & Peptide

CDK9/CyclinT1, GST-tag

SKU : BHP18200224

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Mfr.No.	40307
Description	Human cyclin-dependent kinase 9 (CDK9), also known as C-2K and cell division protein kinase 9, complexed with human cyclin T1 (CCNT1, CycT1), GenBank Accession Nos. NM_001261 (CDK9) and NM_001240 (CyclinT1), a.a. 2- 372(end) (CDK9) and a.a. 2-726(end) (CyclinT1) each with N-terminal GST-tags, co-expressed in a baculovirus-infected Sf9 cell expression system. MW = 69 kDa (CDK9) and 107 kDa (CyclinT1). Host cell line: Sf9 cells Specific activity: 26 pmol/min/ug
Immunogen Region	CDK9: 2- 372(end); CyclinT1: 2-726(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04%Tween-20, 3 mM DTT, 20% glycerol, and1.6 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in 50 uL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 uM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	CDK9: NM_001261; CyclinT1: NM_001240
Mw(kda)	CDK9: 69 kDa; CyclinT1: 107 kDa

Synonyms	cyclin-dependent kinase 9, C-2K, cell division protein kinase 9, and CCNT1 , CycT1
Uniprot	CDK9: P50750; CyclinT1: O60563

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=60%
Storage	At least 6 months at -80°C.

Reference	1. Yang, Z., et al., Nat. 2001; 414: 317-322.2. Baumli, S., et al., EMBO J. 2008; 27(13): 1907-1918.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200224	40307	10 ug	$423.50		Add to Cart

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