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Home / Proteins & Peptide

CDK6/CyclinD3, His-tags

SKU : BHP18200192

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BPS Bioscience

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Mfr.No.	40206
Description	Complex of N-terminal Histaggedrecombinant full-length humanCDK6 (GenBank Accession No.X66365), and N-terminal His tagged,recombinant full length, Cyclin D3.(GenBank Accession No. M90814).Coexpressed in a Baculovirus infectedSf9 cell expression system. CDK6MW=38 kDa, Cyclin D3 MW=33 kDa. Host cell line: Sf9 cells Specific activity: 12 pmol/min/ug
Immunogen Region	full length
Tag	N-terminal His-tags
Formulation	40 mM Tris-HCl, 110 mMNaCl, 2.2 mM KCl, 300 mM Imidazole, 3mM DTT and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 M ATP at 30°C for 60 min. The amount of ATP transferred was calculated using Kinase- Glo reagent (Promega). Avoid freeze/thaw cycles. Storing diluted enzymeis not recommended.
GenBank	NM_001259/NM_001760
Mw(kda)	71 kDa (complex)

Synonyms	Cyclin-Dependent Kinase 6, Cell Devision Kinase 6, CDK6, Cyclin D3, CCD3, G1/S-Specific Cyclin D3, CCND3
Uniprot	Q00534 (CDK6); P30281 (Cyclin D3)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gauduchon J. et al., Int J Cancer. 2008 May 1,122(9):2130-41. 2. Faast R. et al., Oncogene. 2004 Jan 15,23(2):491-502.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200192	40206	20 ug	$390.50		Add to Cart

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