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Home / Proteins & Peptide

CDK5/p35, GST-tag

SKU : BHP18200169

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Mfr.No.	40095
Description	Recombinant full-length human CDK5 (59 kDa) and human p35 (60 kDa), both with N-terminal GST-tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885. host species: Sf9 cells Host species: Sf9 cells Specific activity: 180 pmol/min/ug
Background	CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in Kinase buffer containing 0.2 mM DTT using Histone 1 substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_004935 (CDK5), NM_003885 (p35)
Mw(kda)	108 kDa (complex)

Synonyms	Cyclin-Dependent Kinase 5, Cell Devision Kinase 5, CDK5, p35, Cyclin-Dependent Kinase 5 Regulatory Subunit 1, CDK5R1
Uniprot	Q00535 (CDK5), Q15078 (p35)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Tang, D. et al. Prog Cell Cycle Res. 1996,2:205-16. 2. Dhavan, R. et al. Nat Rev Mol Cell Biol. 2001 Oct,2(10):749-59.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200169	40095	10 ug	$390.50		Add to Cart

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