JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

CDK5/p25, GST-tag

SKU : BHP18200175

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40105
Description	Complex of recombinant full length human CDK5 (GenBank Accession No. NM_004935), a.a. 1-292 (end) with N-terminal GST-tag and p25 (GenBank Accession No. NM_003885), a.a. 99-307 (end) with N-terminal GST-tag. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK5 MW=60 kDa, p25 MW=50 kDa. Host cell line: Sf9 cells Specific activity: 312 pmol/min/ug
Background	CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
Immunogen Region	CDK5 1-292 (end), p25 99-307 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 0.04% Tween-20, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in a Kinase buffer using Histone H1 (BPS cat. #52043) as a substrate with 20 uM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_004935, NM_003885
Mw(kda)	60 kDa (CDK5), 50 kDa (p25)

Synonyms	Cyclin-Dependent Kinase-5, CDK5: PSSALRE p25: CDK5R1; CDK5P35, CDK5R, NCK5A, p23, p35, p35nck5a
Uniprot	Q00535 (CDK5), Q15078 (p35)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16. 2. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200175	40105	10 ug	$390.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.