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Home / Proteins & Peptide

CDK4/CyclinD3, GST-tag

SKU : BHP18200174

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Mfr.No.	40104
Description	Complex of recombinant human CDK4 and CyclinD3, full length, with N-terminal GST-tags (GenBank Accession No. NM_000075, and NM_001760 respectively), co-expressed by baculovirus in Sf9 cells Host cell line: Sf9 cells Specific activity: 14 pmol/min/ug
Background	CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Avoid freeze/thaw cycles.
GenBank	NM_000075/NM_001760
Mw(kda)	64 kDa (CDK4), 59 kDa (Cyclin D3)

Synonyms	Cyclin-Dependent Kinase 4, CDK4: CMM3; PSK-J3; MGC14458 Cyclin D3: CCND3
Uniprot	CDK4: P11802; CyclinD3: P30281

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999. 2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200174	40104	10 ug	$429		Add to Cart

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