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Home / Proteins & Peptide

CDK3/CyclinE1, GST-tag

SKU : BHP18200173

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BPS Bioscience

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Mfr.No.	40103
Description	Complex of N-terminal GST-tagged recombinant full length human CDK3 (GenBank Accession No. NM_001258), and Cyclin E1 (GenBank Accession No. NM_001238). Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK3 MW=61 kDa, Cyclin E1 MW=74 kDa. Host cell line: Sf9 cells Specific activity: 30 pmol/min/ug
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Species	Human
Application	Useful for three dimensional structure studies, binding assays, enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 uM ATP at 30°C for 50 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_001258 (CDK3), NM_001238 (Cyclin E)
Mw(kda)	135 kDa (complex)

Synonyms	Cyclin-Dependent Kinase 3, Cell Devision Kinase 3, CDK3, Cyclin E1, CCN1, CCNE1
Uniprot	Q00526 (CDK3), P24864 (Cyclin E)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Bullrich, F. et al., Cancer Res. (1995); 55:1199-1205. 2. Ye, X. et al., Proc. Nat. Acad. Sci. (2001); 98:1682-1686.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200173	40103	10 ug	$390.50		Add to Cart

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