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Home / Proteins & Peptide

CDK2 (no tag)/CyclinA2, His-GST-tags

SKU : BHP18200299

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BPS Bioscience

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Mfr.No.	41101
Description	Complex of recombinant full-length human CDK2 (GenBank accession No. NM_001798), a.a. 1-298 (end), MW = 34 kDa with no tag, and CyclinA2 (GenBank accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 57 kDa. Both were co-expressed in a baculovirus Sf9 insect cell expression system. Host cell line: Sf9 cells Specific activity: 203 pmol/min/ug
Immunogen Region	CDK2 a.a. 1-298 (end), CyclinA2 a.a. 174-432 (end)
Tag	CDK2 no tag, CyclinA2 N-terminal His-GST-tags
Formulation	20 mM NaH₂PO₄/Na₂HPO₄, pH 8.0, 400 mM NaCl, 20 mM glutathione, 10% glycerol, 2 mM DTT, and 1 mM EDTA
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 uM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_001798 (CDK2), NM_001237 (CyclinA2)
Mw(kda)	91 kDa (complex)

Synonyms	Cyclin-Dependent Kinase 2, Cell Devision Kinase 2, CDK2, Cyclin A2, CCN1, CCNA2
Uniprot	P24941 (CDK2), P20248 (CyclinA2)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9. 2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200299	41101	10 ug	$390.50		Add to Cart

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