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Home / Proteins & Peptide

CDK2/CyclinA2, GST-tag

SKU : BHP18200172

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BPS Bioscience

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Mfr.No.	40101
Description	Recombinant full-length human CDK2 (GenBank Accession No. NM_001798), a.a. 2-298 (end) with N-terminal GST-tag, MW = 61 kDa, and CyclinA2 (GenBank Accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 75 kDa. Both proteins were coexpressed in a baculovirus Sf9 insect cell expression system. Host cell line: Sf9 cells Specific activity: 390 pmol/min/ug
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 uM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_001798 (CDK2), NM_001237 (CyclinA2)
Mw(kda)	136 kDa (complex)

Synonyms	Cyclin-Dependent Kinase 2, Cell Devision Kinase 2, CDK2, Cyclin A2, CCN1, CCNA2
Uniprot	P24941 (CDK2), Q13825 (CyclinA2)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9. 2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200172	40101	10 ug	$390.50		Add to Cart

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