Complex of human CDK13 (cyclin dependent kinase 13) and human cyclin K. The proteins were co-expressed in Sf9 insect cells along with S. cerevisiae CAK1 (Serine/threonine-protein kinase CAK1).Recombinant human CDK13, encompassing amino acids 516-1512 (end), contains an N-terminal GST-tag. Recombinant human Cyclin K, full length encompassing amino acids 1-580 (end), also contains an N-terminal GST-tag. Recombinant S. cerevisiae CAK1, encompassing amino acids 2-368 (end), contains a FLAG-tag. The proteins were purified using the GST tags. Constructs: CDK13 (GST-516-1512(end)) / Cyclin K (GST-2-580(end))
Immunogen Region
CDK13: 516-1512(end); Cyclin K: 2-580(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Species
Human
Notes
Avoid freeze/thaw cycles.
GenBank
CDK13: NM_003718; Cyclin K: NM_001099402
Mw(kda)
CDK13: 138 kDa; Cyclin K: 90 kDa
Synonyms
CHED, CDC2L, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, CDC2L5, CHDFIDD, Cell division protein kinase 13, hCDK13, CCNK, CPR4, IDDHDF, CAK-1, CDK-activating kinase, 101128-1, 101128-2