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Home / Proteins & Peptide

CDK1/CyclinB1, GST-tag

SKU : BHP18200251

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BPS Bioscience

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Mfr.No.	40454
Description	Human CDK1/CyclinB1complex, also known as CDC and CCNB1,CCNB respectively. GenBank AccessionNos. NM_001786 and NM_031966respectively, full-length with N-terminal GST-tagson both proteins, expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 60 kDa (CDK1) MW = 75 kDa(CyclinB1). Host cell line: Sf9 cells Specific activity: 90 pmol/min/ug
Immunogen Region	Full-length
Tag	N-terminal GST-tag
Formulation	40 mM Tris pH 8.0, 110 mMNaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mMDTT and 20% glycerol.
Species	Human
Application	Useful for studying enzyme kinetics and screening inhibitors.
Notes	Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 uM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank	CDK1: NM_001786; CyclinB1: NM_031966
Mw(kda)	CDK1: 60kDa; CyclinB1: 75kDa

Synonyms	Cyclin-dependent kinase 1, CDC and CCNB1, CCNB respectively
Uniprot	CDK1: P06493; CyclinB1: P14635

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Vantieghem, A. et al., J. Biol. Chem. 2002; 277(40): 37718-37731. 2. Rigas, A.C. et al., Oncogene. 2007; 26(55): 7611-7619.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200251	40454	10 ug	$423.50		Add to Cart

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