Mouse CD73, also known as 5'-nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_011851, a.a. 29-549 with C-terminal Avi-His-tag, expressed in a HEK293 cell expression system. MW = 61 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Background
CD73, or cluster of differentiation 73, is an enzyme which hydrolyzes AMP to adenosine. Increased concentrations of adenosine in the microenvironment has an immunosuppressive effect and promotes tumor cell growth.
Immunogen Region
29-549
Tag
Avi-His-Tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Mouse
Application
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes
The aggregation state of this product has been confirmed to be<10% Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).