Human CD73, also known as 5'-nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_002526, a.a. 27-547 with C-terminal Avi-His-tag, expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag technology. MW = 61 kDa.
Specific activity: >20 nmol/min/ug
Immunogen Region
27-547
Tag
C-terminal Avi-His-Tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done in 50 uL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 uM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
GenBank
NM_002526
Mw(kda)
61 kDa
Synonyms
5'-Nucleotidase, Ecto-5'-Nucleotidase
Uniprot
P21589
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.