Mouse CD73, also known as 5'-nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_011851, a.a. 29-549 with C-terminal Avi-His-tag, expressed in a HEK293 cell expression system. MW = 61 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag technology.
Immunogen Region
29-549
Tag
C-terminal Avi-His-Tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Mouse
Notes
HiP indicates a high purity protein with>=90% purity as measured by gel filtration. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_011851
Mw(kda)
61 kDa
Synonyms
5'-nucleotidase, Ecto-5'-nucleotidase
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution.
Storage
At least 6 months at -80°C.
Reference
1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.