Human CD48, Fc fusion protein, also known as Cluster of Differentiation 48, BLAST-1, or SLAMF2. GenBank Accession No. NM_001778, a.a. 27-220 expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag technology. MW = 51 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
27-220
Tag
C-terminal Avi-tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for studying protein binding and screening small molecular inhibitors
Notes
HiP indicates a high purity protein with>=90 percent purity by gel filtration. This protein runs at a higher MW by SDS-PAGE due to glycosylation. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_001778
Mw(kda)
51 kDa+glycans
Synonyms
Cluster of Differentiation 48, BLAST-1, SLAMF2, CD2L
Uniprot
P09326
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Nakajima, H. et al., European Journal of Immunology. 1999; 29(5): 1676-1683.2. Brown, M.H., et al., Journal of Experimental Medicine. 1998; 188(11): 2083.