Human CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1, and enzymatically biotin-labeled using Avi-tag technology. MW = 42.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
19-139
Tag
C-terminal Fc fusion (Human IgG1)
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Notes
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_001777
Mw(kda)
42.2 kDa+glycans
Synonyms
leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6
Uniprot
Q08722
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C (for all proteins)
Reference
1. Vernon-Wilson, E.F., et al., Eur. J. Immunol. 2000; 30(8): 2130-2137. 2. Majeti, R. et al., Cell. 2009; 138(2): 286-299.