Mouse CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. NM_010581.3, a.a. 19-158 fused at the C-terminus to the Fc portion of human IgG1 and C-terminal Avi-tag expressed in a HEK293 cell expression system. The protein is enzymatically biotinylated using AviTag technology. MW = 44 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
19-158
Tag
C-terminal Fc fusion (Human IgG1), C-terminal Avi-Tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Mouse
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Notes
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_010581.3
Mw(kda)
44 kDa+glycans
Synonyms
leukocyte surface antigen CD47, Rh-related antigen, CD47, CD-47, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6
Uniprot
Q08722
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C (for all proteins)
Reference
1. Vernon-Wilson, E.F., et al., Eur. J. Immunol. 2000; 30(8): 2130-21375.2. Majeti, R. et al., Cell. 2009; 138(2): 286-299.