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Home / Proteins & Peptide

CD45(PTPRC), His-tag

SKU : BHP18200039

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Mfr.No.	30044
Description	Protein tyrosine phosphatase CD45, also known as PTPRC (GenBank accession No. NM_080922), catalytic domain, a.a. 632-1304 (end) with N-terminal His-tag, MW=81 kDa, expressed in a Baculovirus Sf9 expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 341 pmole/min/ug
Background	PTPRC (CD45) contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
Immunogen Region	632-1304
Tag	N-terminal His-tag
Formulation	40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Notes	20 mM HEPES, pH 7.2,100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1mM PNPP, 20 min at room temperature.Absorbance was measured at 405 nm.Specific activity was calculated based onpnp=18,000M-1cm-1. Avoid freeze/thaw cycles.
GenBank	NM_002838
Mw(kda)	81 kDa

Synonyms	CD45, LCA, T200, LY5, B220, GP180
Uniprot	P08575

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	>12 months at -80°C

Reference	1. Rachmilewitz, J. et al., J. Biol. Chem. 278, 14059-14065. 2. Fernandis, A.Z. et al., J. Biol. Chem. 278, 9536-9543.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200039	30044	50 ug	$357.50		Add to Cart

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