Human CD39, also known as Cluster of Differentiation 39, GenBank Accession number NM_001776, a.a. 38-478 with C-terminal His-tag. MW = 51 kDa, expressed in an HEK293 expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Specific activity: >=1300 pmol/min/ug
Immunogen Region
38-478
Tag
C-terminal His-tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Add CD39 enzyme to 50 ul reaction mix containing 0.5 mM ATP and 10 mM CaCl2. Incubate 1h at RT with slow shaking. Add 50 ul GLO Max to every well. Finally, read luminescence.