Recombinant human CD38 (CD38 molecule) encompassing amino acids 43 to300 (end). This construct contains a C-terminal His-tag (6xHis). Therecombinant protein was affinity purified and labeled with APC.
Immunogen Region
43-300
Tag
APC Labeled, C-terminal His-Tag
Formulation
8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Species
Human
Application
Useful for studying protein binding and for screening small molecules and antibodies
Notes
Add CD38 enzyme to 50 ul reaction mix containing NGD+ (Nicotinamide Guanine Dinucleotide). Incubate 4 min at room temperature. Read fluorescence (lambda exc=300 nm, lambda em=410 nm).
Protect from light. Avoid freeze/thaw cycles.
GenBank
NM_001775
Mw(kda)
31 kDa
Synonyms
ADPRC1; ADPRC 1, CD38
Uniprot
P28907
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Damle, R. et al., Blood. 1998; 94: 1840-1847.2. Howard, M. et al., Science. 1993; 262(5136): 1056-1059.