Human CD28 homolog (CD28H), also known as Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) and immunoglobulin and prolinerich receptor 1 (IGPR-1). GenBank Accession No. NM_144615, a.a. 23-128 fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal AviTag expressed in a HEK293 expression system. MW = 40 kDa. This protein is enzymatically biotinylated using Avi-tag technology. Biotinylation is confirmed to be greater than 90%. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
23-128
Tag
C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for studying protein binding and for screening small molecules and antibodies.
Notes
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_144615
Mw(kda)
40 kDa
Synonyms
CD28 homolog, Transmembrane and immunoglobulin domain-containing protein 2 TMIGD2, immunoglobulin and proline-rich receptor 1, IGPR-1
Uniprot
P10747
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Zhu, Y., et al., Nat. Commun. 2013; 4:2043. 2. Zhu, Y., et al., J. Immunol. 2015; 194:142.2.