Human CD155, also known as Poliovirus receptor and Nectin-like protein 5, GenBank Accession No. NM_006505, a.a. 27-343 with a C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 35 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
27-343
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for studying protein binding and for screening small molecules and antibodies.
Endotoxin Level
<1 EU/ug
Notes
CD155, His-tagbinding to TIGIT was confirmed according tothe protocol for BPS Cat. 72029. Briefly, 4ng of TIGIT-biotin was mixed with varyingamounts of CD155, His-tag in TIGIT assaybuffer at a final volume of 10 ul and allowedto incubate for 1 hr at room temp. Nickleacceptor beads were added for 30 min,followed by Strep-conjugated acceptorbeads for 30 min and reading alpha-counts.
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_006505
Mw(kda)
35 kDa
Synonyms
Poliovirus receptor (PVR) and Nectin-like protein 5
Uniprot
P15151
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Bottino, C. et al., J. Exp. Med. 2003; 198(4): 557-567. 2. Pende, D. et al., Blood. 2005; 105(5): 2066-2073.