Human secreted CD137, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, and ILA, GenBank Accession No. NM_001561, a.a. 24-186, expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1 and enzymatically biotin-labeled using Avi-tag technology. MW = 45.9 kDa (monomer). Biotinylation is confirmed to be>90%. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
24-186
Tag
C-terminal Fc fusion, Avi-Tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for studying protein binding and screening small molecular inhibitors
Endotoxin Level
<1 EU/ug
Notes
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_001561
Mw(kda)
45.9 kDa + glycans
Synonyms
Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, ILA
Uniprot
Q07011
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Wilcox, R.A., et al., J. Clin. Invest. 2002; 109(5): 651-659.2. Foell, J. et al., J. Clin. Invest. 2003; 111(10): 1505-1518.