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Home / Proteins & Peptide

CBL, GST-Tag

SKU : BHP18200945

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BPS Bioscience

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Mfr.No.	100370
Description	Human CBL, also known as CBL-2, Cbl Proto-Oncogene, Signal Transduction Protein CBL, and Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase, Genbank Accession No. NM_005188, a.a. 49-434 with N-terminal GST-tag, expressed in an E. coli expression system. MW = 71 kDa.
Immunogen Region	49-434
Tag	N-terminal GST-Tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween20, 20% glycerol, 16 mM glutathione, 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Notes	10 uL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 0.2 uM Ub(Biotin), 50 nM Ube1, 1 mM ATP, 2 uM of UBCH5b, and various amount of CBL-B and C-CBL. The reaction was initiated with the addition of ATP, and incubated at 30°C for 3 hours. Tb labeled anti-GST antibody donor and xl665 labeled acceptor was added and incubated at room temperature for one hour. Avoid freeze/thaw cycles.
GenBank	NM_005188
Mw(kda)	71 kDa

Synonyms	CBL-2, Cbl Proto-Oncogene, Signal Transduction Protein CBL; Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase, c-CBL
Uniprot	P22681

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	Griffiths, E.K., Molec. and Cellular Bio. 2003; Oct. 23(21):7708-7718Keane, M.M., Oncogene, 1999 June; 18: 3365-3375.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200945	100370	100 ug	$379.50		Add to Cart

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