Recombinant human CBLB (Cbl proto-oncogene B), encompassing amino acids 39-426. This construct contains an N-terminal GST-tag followed by a PreScission Protease Cleavage site to facilitate removal of the GST portion and contains the mutation of interest (Y363E). The recombinant protein was affinity purified.
host species: E. coli
Host species: E. coli
Immunogen Region
39-426
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, and variable glutathione
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
Assay was performed according to the protocol in CBL-B TR-FRET Assay Kit (Biohippo #79575).
Avoid freeze/thaw cycles.
GenBank
NM_170662
Mw(kda)
72 kDa
Synonyms
E3 ubiquitin-protein ligase CBL-B, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, RING-type E3 ubiquitin transferase CBL-B, SH3-binding protein CBL-B, Signal transduction protein CBLB, RNF56, Nbla00127