Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(a.a. 1-16) + cathepsin V (a.a. 18-334), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
Specific activity: >=250 pmol/min/ug
Immunogen Region
CD33 1-16, CathepsinV 18-334
Tag
C-terminal His-tag
Formulation
40 mM MES pH 5.0, 120 mM NaCl and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay is conducted in 25 mM MES buffer pH5.0, 0.1 M NaCl, 5 mM DTT and 20 uM substrate (Z-LeuArg-AMC) at room temperature for 30 minutes. Fluorescence intensity is measured at exc360/em460.
Avoid freeze/thaw cycles.
GenBank
NM_001333.2
Mw(kda)
37 kDa
Synonyms
CTSV
Uniprot
O60911
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Puzer L. et al., Biochem. Biol. Chem. 389(2), 195-200 (2008). 2. Burden R.E. et al., Biol. Chem. 388 (5), 541-545 (2007).