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Home / Proteins & Peptide

Cathepsin B, Human

SKU : BHP18500459

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iXCells Biotechnologies

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Mfr.No.	IX03322, IX03322-10
Description	Cathepsin B is an enzymatic protein that is a member of the peptidase (or protease) family. Elevated levels of cathepsin B occur in a wide variety of disease, causing numerous pathologies including cell death, inflammation, and the production of toxic peptides. For example, neuroscience research suggests that cathepsin B plays a role in inducing epilepsy resulting in a significant amount of cell death. Seizure-induced rats treated with a cathepsin B inhibitor exhibited improved neurological scores and learning ability with reduced neuronal cell death.Recombinant Human Cathepsin B produced in CHO cells is a polypeptide chain containing 332 amino acids. A fully biologically active molecule, rhCathepsin B has a molecular mass of 43 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Formulation	Liquid after a 0.2 um filtered solution in 25 mM Tris, 150 mM NaCl, 20% Glycerol, pH 8.0.
Molecular Weight	43 kDa, observed by reducing SDS-PAGE.
Source	CHO
Accession Number	P07858
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	The Specific Activity is 500 pmol/min/ug, measured by Cathepsin B s ability to cleave the fluorogenic peptide substrate Z-FR-AMC (Enzo, Catalog: P139).Activation Buffer: 25 mM MES, 5 mM DTT, pH 5.0 Assay Buffer: 25 mM MES, pH 5.0Activate Cathepsin B: Dilute Cathepsin B to 10 ug/mL in Activation Buffer and incubate at room temperature for 15 minutes.Incubate10 uM Z-FR-AMC and 0.2ng/uL activated Cathepsin B at room temperature for 5 minutes. Quantified by releasing the AMC which is detected by at excitation and emission wavelengths of 360 nm and 460 nm, respectively.

Synonyms	Cathepsin B; APP secretase; APPS
Sequence	RSRPSFHPLS DELVNYVNKR NTTWQAGHNF YNVDMSYLKRLCGTFLGGPK PPQRVMFTED LKLPASFDAR EQWPQCPTIKEIRDQGSCGS CWAFGAVEAI SDRICIHTNA HVSVEVSAEDLLTCCGSMCG DGCNGGYPAE AWNFWTRKGL VSGGLYESHVGCRPYSIPPC EHHVNGSRPP CTGEGDTPKC SKICEPGYSPTYKQDKHYGY NSYSVSNSEK DIMAEIYKNG PVEGAFSVYSDFLLYKSGVY QHVTGEMMGG HAIRILGWGV ENGTPYWLVANSWNTDWGDN GFFKILRGQD HCGIESEVVA GIPRTDQYWE KIHHHHHHHH HH

Purity	95% as analyzed by reducing SDS-PAGE.
Storage	Recombinant Human Cathepsin B remains stable up to 6 months at lower than -70°C from date of receipt under sterile conditions. Up to 3 months at lower than -70°C under sterile conditions after opening. Avoid repeated freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500459-1	IX03322-10	10ug	$182		Add to Cart

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