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Home / Proteins & Peptide

Cathepsin B, His-tag

SKU : BHP18200776

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BPS Bioscience

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Mfr.No.	80001
Description	Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide (a.a. 1-16) + CathepsinB (a.a. 18-339), with C-terminal His-tag, MW=43 kDa, expressed in FreeStyle 293-F cells. Specific activity: >= 4048 pmol/min/ug
Immunogen Region	CD33 1-16, CathepsinB 18-339
Tag	C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	25 mM MES, pH 5.0, 10 uMsubstrate (Z-Leu-Arg-AMC), 10 min, roomtemperature. Prior to assay enzyme wasactivated with preincubation at 10 ug/ml in 25mM MES, pH 5.0, 5 mM DTT for 15 min at RT. Avoid freeze/thaw cycles.
GenBank	NM_001908
Mw(kda)	43 kDa

Synonyms	Amyloid Precursor Protein Secretase, CTSB, APP Secretase
Uniprot	P07858

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Tu, C. et al., Cancer Res. 2008 Nov 15;68(22):9147-56. 2. Hwang, JH. et al., Cancer Lett. 2009 Jan18;273(2):266-72.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200776	80001	10 ug	$445.50		Add to Cart

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