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Home / Proteins & Peptide

Caspase3, His-tag

SKU : BHP18201764

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Mfr.No.	80500-1
Description	Human caspase 3 (CASP3), also known as CPP32 and apopain, a.a. 1 - 277 (end) (GenBank Accession No. NM_004346, var alpha) with C-terminal His-tag, expressed in an E. coli expression system. Procaspase-3 is self-cleaved into two subunits, MW = 17 kDa and 13 kDa, to form the active heterotetrameric complex. Specific activity: >= 6,784 pmol/min/ug
Background	Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.
Immunogen Region	1 - 277 (end)
Tag	C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's Homogeneous Caspase-3 Assay Kit, Cat. # 80700. Assay conditions: 10 mM HEPES, pH7.4, 10 mM EDTA, 0.05% Chaps, 5 mMDTT, and 20 uM Caspase 3 substrate.Incubate for 30 minutes at roomtemperature. Fluorescence intensity ismeasured at exc380/em500 nm. Avoid freeze/thaw cycles.
GenBank	NM_004346
Mw(kda)	see Description

Synonyms	caspase-3, CASP3, caspase 3, CPP32, apopain, Yama
Uniprot	P42574

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Lee, D., et al., J Biol Chem. 2000 May 26;275(21):16007-14 2. Suzuki, Y., et al., Proc Natl Acad Sci USA. 2001 Jul 17;98(15):866-7.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201764	80500-1	10 ug	$313.50		Add to Cart

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